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GeneKam Biotechnology AG PCR檢測(cè)試劑盒
Genekam Biotechnology AG公司簡(jiǎn)介
Genekam Biotechnology AG成立于1999年,從2004年至2015年已向全球70個(gè)國(guó)家提供PCR檢測(cè)試劑盒。
Genekam Biotechnology AG公司技術(shù)領(lǐng)域
Genekam檢測(cè)產(chǎn)品涵蓋流感病毒、中東呼吸綜合征病毒(MERS)、丙型肝炎病毒、登革熱、皰疹病毒、分枝桿菌、衣原體、支原體、沙門氏菌等食品病原體等。
Genekam致力于為大流行性病毒的預(yù)防、檢測(cè)和治療提供解決方案。
細(xì)胞培養(yǎng)基中2-3%的胎牛血清足以促進(jìn)人單核細(xì)胞培養(yǎng)物的發(fā)育和生長(zhǎng)
目的:通常的做法是,10-20%的胎牛血清(FCS)被用于細(xì)胞培養(yǎng)基中,用于人單核細(xì)胞以及其他細(xì)胞培養(yǎng)物的發(fā)育和維持。FCS是通過(guò)涉及動(dòng)物的痛苦過(guò)程產(chǎn)生的,是主要的成本因素之一。FCS數(shù)量的減少可以減少其不道德行為,并大大降低成本。
材料與方法: 因此,將2-3%的FCS和10%的FCS用于開(kāi)發(fā)不同的單核細(xì)胞培養(yǎng)物進(jìn)行比較,并測(cè)試了不同的參數(shù),如形態(tài)學(xué)、抗體的產(chǎn)生、用微型柱和磁珠分離DNA/RNA細(xì)胞以及人類內(nèi)部對(duì)照的存在與實(shí)時(shí)PCR。
結(jié)果:結(jié)果發(fā)現(xiàn),在上述參數(shù)中,2-3%的FCS和10%含FCS的培養(yǎng)基之間沒(méi)有差異。開(kāi)發(fā)了一百多種這樣的細(xì)胞培養(yǎng)物,其中許多細(xì)胞培養(yǎng)物維持了 3 年以上。
結(jié)論:2-3%的FCS足以長(zhǎng)期開(kāi)發(fā)和維持人類單核細(xì)胞培養(yǎng)物。
Genekam Biotechnology AG公司熱銷產(chǎn)品
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
Parainfluenza virus 1,2,3 Conventional PCR kit | K062 | 100reactions |
Human Interleukin-10real time PCR kit | FR415 | 100reactions |
Magnetic Separation VEGFR2/CD39 Positive Cells (endothelial) (only magnetic beads) | STEM60 | 10 reactions |
Polio Virus (human) Conventional PCR kit | K039 | 100reactions |
Human Interleukin-13real time PCR kit | FR416 | 100reactions |
Magnetic CD31 Positive Cells (endothelial) (only magnetic beads) | STEM61 | 10 reactions |
PRRSV (porcine reproductive and respiratory syndrome virus) Conventional PCR kit | K042 | 100reactions |
Human Interleukin-23-alfareal time PCR kit | FR422 | 100reactions |
Magnetic bead antibody isolation | SB0142 | 2 reactions |
Rotavirus Conventional PCR kit | K060 | 100reactions |
Human Interleukin-5real time PCR kit | FR414 | 100reactions |
Magnetic bead antibody isolation | SB0143 | 10 reactions |
Rubella Virus (human) Conventional PCR kit | K838 | 100reactions |
Human Interleukin-6real time PCR kit | FR421 | 100reactions |
Endotoxin Removal Kit | SB0146A | 1 ml |
Sandfly fever sicilian virus Conventional PCR kit | K249 | 100reactions |
Human Interleukin-8real time PCR kit | FR420 | 100reactions |
Endotoxin Removal Kit | SB0146B | 5 ml |
Tick-borne encephalitis TBE (human) Conventional PCR kit | K034 | 100reactions |
Human Papilloma virus real time PCR kit | FR039 | 100reactions |
Endotoxin Removal Kit | SB0146C | 10 ml |
Universal Fluhunter 2: Universal PCR kit for Hemagglutinin gene (HA-gene) decoding and detection of influenza viruses + 10 genesequencings Conventional PCR kit | K336 | 100reactions |
Human Papilloma Virus HPV-16 (high risk profile) real time PCR kit | FR041 | 100reactions |
Erythrocyte removal kit (Sample) | SB0147 | 10 ml |
Yellow fever YF Conventional PCR kit | K412 | 100reactions |
Human Papilloma Virus HPV-18 (high risk profile) real time PCR kit | FR042 | 100reactions |
Erythrocyte removal kit | SB0147S | 100 ml |
Arthropod-Borne Universal Flavivirus kit (It detects Dengue virus, West nile virus, JEV, TEBV, Yellow fever, St. Luis Encephalitis Virus and other flaviviruses of athropod like Mosquitoes an... Conventional PCR kit | K267 | 100reactions |
Human Papilloma virus-HPV High Risk Genotyping (16,18,31,33) real time PCR kit | FR214 | 100reactions |
Erythrocyte removal kit | SB0147M | 200 ml |
Fluhunter: Ready to use PCR kit for H3N2 (Iowa strain) Conventional PCR kit | K313 | 100reactions |
Human Papilloma virus-HPV-33,51, 52 and 58real time PCR kit | FR370 | 100reactions |
Human T Cell( CD4 Positive Cells ) magnetic beads isolation Kit (full kit) | SB0149 | 10 reactions |
Fluhunter -Corona (Universal Kit) with 5 genesequencings Conventional PCR kit | K354 | 100reactions |
Human Papilloma virus-HPV-35-53, 59 and 66real time PCR kit | FR371 | 100reactions |
Human T Cell ( CD8 Positive Cells ) magnetic beads isolation Kit (full kit) | SB0150 | 10 reactions |
Fluhunter -Corona (Universal Kit) with 25 genesequencings Conventional PCR kit | K355 | 100reactions |
Human Papilloma virus-HPV-39real time PCR kit | FR373 | 100reactions |
Human T Cell ( CD3 Positive Cells ) magnetic beads isolation Kit (full kit) | SB0151 | 10 reactions |
Fluhunter-Corona Plus (Universal kit) with 5 genesequencings Conventional PCR kit | K356 | 100reactions |
Human Papilloma virus-HPV-39, 45, 56 and 68real time PCR kit | FR369 | 100reactions |
Human B Cell ( CD19 Positive Cells ) magnetic beads isolation Kit (full kit) | SB0152 | 10 reactions |
Fluhunter-Corona Plus (Universal kit) with 50 genesequencings Conventional PCR kit | K358 | 100reactions |
Human Papilloma virus-HPV-45real time PCR kit | FR374 | 100reactions |
Human B Cell ( CD20 Positive Cells ) magnetic beads isolation Kit (full kit) | SB0153 | 10 reactions |
Fluhunter Middle East SARS virus (Single Check):Ready to use PCR Kit to detect the middle east SARS virus Conventional PCR kit | K360 | 100reactions |
Human Papilloma virus-HPV-52real time PCR kit | FR375 | 100reactions |
Human B Cell ( CD27 Positive Cells ) magnetic beads isolation Kit (full kit) | SB0154 | 10 reactions |
Fluhunter Middle East SARS virus (Double Check):Ready to use PCR Kit to detect the middle east SARS virus Conventional PCR kit | K361 | 100reactions |
Human Papilloma virus-HPV-6 and 11real time PCR kit | FR372 | 100reactions |
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